Nesta pesquisa, estudamos os custos de transporte por consulta ambulatorial em pacientes referenciados para avaliação cirúrgica especializada em um hospital terciário no Rio de Janeiro, Brasil. Trata-se de um estudo descritivo com responsáveis por pacientes pediátricos agendados para consulta de cirurgia pediátrica, questionando fatores ligados ao custo de transporte e de oportunidade (perda de remuneração, alimentação, pagamento de cuidadores para outros filhos, despesas para outros acompanhantes). Aproximadamente metade dos pacientes tinham até 5 anos de idade, cerca de 2/3 apresentavam doenças de resolutividade cirúrgica simples e definitiva e 181 famílias (89,17%) contavam com renda familiar mensal de até R$ 1.999,00. A proporção de famílias beneficiadas por transporte gratuito variou entre 4,26-15,56% para pacientes morando até 100km de distância do hospital (45,83% para pacientes com residência a mais de 100km da instituição). Dos responsáveis, 176 (87,13%) relataram despesas para alimentação, 12 (5,94%) pagavam cuidadores para os outros filhos no dia da consulta e 80 (39,6%) referiram perda do pagamento do dia de trabalho. Dos casos de alta complexidade, 9,33% das mães entrevistadas abriram mão de exercer atividade remunerada regular. As despesas com transporte para uma consulta em cirurgia pediátrica em um hospital de referência do Rio de Janeiro custam em média 4,42% do salário mínimo vigente, com uma média de 217,32 minutos de deslocamento por consulta. Despesas com alimentação e perda de remuneração pela ausência no trabalho também implicam encargos financeiros ou perda de remuneração significativos para o paciente em cada consulta.

En esta investigación se estudiaron los costes de transporte por visita ambulatoria en pacientes remitidos para evaluación quirúrgica especializada en un hospital de atención terciaria en Río de Janeiro, Brasil. Es un estudio descriptivo con responsables de pacientes pediátricos programados para consulta de cirugía pediátrica, en el que se cuestionaron factores relacionados con el coste del transporte y los costes de oportunidad (pérdida de remuneración, alimentación, pago de cuidadores de otros niños, gastos de otros cuidadores). Aproximadamente la mitad de los pacientes tenían hasta 5 años de edad; cerca de 2/3 presentaban enfermedades de resolución quirúrgica simple y definitiva, 181 familias (89,17%) con renta familiar mensual de hasta BRL 1.999,00. La proporción de familias que se benefician de transporte gratuito varía entre el 4,26% y el 15,56% para los pacientes que viven hasta a 100 km del hospital (45,83% para los pacientes que viven a más de 100 km de la institución). Ciento setenta y seis (87,13%) cuidadores declaran gastos de alimentación, 12 (5,94%) pagan a los cuidadores de sus otros hijos en el día de la consulta y 80 (39,6%) declaran pérdida de salario por la jornada laboral. De los casos de alta complejidad, el 9,33% de las madres entrevistadas habían abandonado su actividad remunerada habitual. Los gastos de transporte para una consulta de cirugía pediátrica en un hospital de referencia de Río de Janeiro cuestan de media el 4,42% del salario mínimo vigente, con una media de 217,32 minutos de tiempo de viaje/consulta. Los gastos de alimentación y la pérdida de salario por ausencia en el trabajo también implican una importante carga económica o pérdida de salario para el paciente en cada cita.

In this study, we investigated the costs of transportation for each outpatient consultation in patients referred to specialized surgical evaluation in a tertiary hospital in Rio de Janeiro, Brazil. Descriptive study with guardians of pediatric patients scheduled for pediatric surgery, questioning transportation cost, and opportunity costs (loss of remuneration, food, payment of caregivers for other children, expenses for other companions). About half patients were aged up to five years; about two thirds had diseases of simple and definitive surgical resolution; and 181 families (89.17%) presented monthly family income up to BRL 1,999.00. The proportion of families that benefited from free transportation ranged from 4.26% to 15.56% for patients living up to 100 km away from the hospital (45.83% for patients living more than 100 km away from the institution). A total of 176 (87.13%) guardians reported expenses buying food, 12 (5.94%) paid caregivers to the other children on the day of the consultation, and 80 (39.6%) reported loss of remuneration for the working day. Among the high complexity cases, 9.33% of the mothers have given up their regular paid employment. Transportation expenses for a pediatric surgery consultation at a reference hospital in Rio de Janeiro cost about 4.42% of the current minimum wage, spending around 217.32 minutes of displacement/consultation. Expenses with food and loss of remuneration due to absence at work also entail significant financial charges or loss of remuneration for the patient at each consultation.

Complete understanding of the local stress triaxiality and stress concentration is essential to ensuring structural safety of several structures. A combination of mechanical tests with numerical simulations can be used to obtain this information. One way to study stress triaxiality is by modifying the standard tensile test geometry (ASTM E8) with a notch. Based on previous results from the literature, five notches were chosen: 10, 5, 3, 2, and 1 mm. These geometries were tested, and the results were numerically reproduced using the Abaqus/Explicit 2020 software. The models used were a non-linear model with the Gurson-Tvergaard-Needleman damage model to reproduce the failure. The numerical analyses allowed the assessment of the von Mises stress and stress triaxiality near the notch to compare with the standard smooth specimen. Two instants were considered as crack propagation onset; the instant of the maximum von Mises stress in the element at the center of the specimen, where the failure process begins; and the moment of maximum stress in the true stress x true strain curve. For the von Mises stress analysis, the difference between the curves was small. The stress triaxiality is a better variable to visualize the influences of the notch. When the strain is equal to a 0.07 (instant of the maximum force for the standard specimens), for the smaller notches (1 and 2 mm), there is a region where the effective plastic strain is zero. Consequently, the stress triaxiality is larger in this region than in the center. For the crack propagation onset instant, the plastic strain occurs along the whole transversal section. In this instant, the maximum value of stress triaxiality occurs in the center for all specimens. These results demonstrate that the stress triaxiality changes as the strain increases, i.e., varies with time.

In this study, we investigated the costs of transportation for each outpatient consultation in patients referred to specialized surgical evaluation in a tertiary hospital in Rio de Janeiro, Brazil. Descriptive study with guardians of pediatric patients scheduled for pediatric surgery, questioning transportation cost, and opportunity costs (loss of remuneration, food, payment of caregivers for other children, expenses for other companions). About half patients were aged up to five years; about two thirds had diseases of simple and definitive surgical resolution; and 181 families (89.17%) presented monthly family income up to BRL 1,999.00. The proportion of families that benefited from free transportation ranged from 4.26% to 15.56% for patients living up to 100 km away from the hospital (45.83% for patients living more than 100 km away from the institution). A total of 176 (87.13%) guardians reported expenses buying food, 12 (5.94%) paid caregivers to the other children on the day of the consultation, and 80 (39.6%) reported loss of remuneration for the working day. Among the high complexity cases, 9.33% of the mothers have given up their regular paid employment. Transportation expenses for a pediatric surgery consultation at a reference hospital in Rio de Janeiro cost about 4.42% of the current minimum wage, spending around 217.32 minutes of displacement/consultation. Expenses with food and loss of remuneration due to absence at work also entail significant financial charges or loss of remuneration for the patient at each consultation.

En esta investigación se estudiaron los costes de transporte por visita ambulatoria en pacientes remitidos para evaluación quirúrgica especializada en un hospital de atención terciaria en Río de Janeiro, Brasil. Es un estudio descriptivo con responsables de pacientes pediátricos programados para consulta de cirugía pediátrica, en el que se cuestionaron factores relacionados con el coste del transporte y los costes de oportunidad (pérdida de remuneración, alimentación, pago de cuidadores de otros niños, gastos de otros cuidadores). Aproximadamente la mitad de los pacientes tenían hasta 5 años de edad; cerca de 2/3 presentaban enfermedades de resolución quirúrgica simple y definitiva, 181 familias (89,17%) con renta familiar mensual de hasta BRL 1.999,00. La proporción de familias que se benefician de transporte gratuito varía entre el 4,26% y el 15,56% para los pacientes que viven hasta a 100 km del hospital (45,83% para los pacientes que viven a más de 100 km de la institución). Ciento setenta y seis (87,13%) cuidadores declaran gastos de alimentación, 12 (5,94%) pagan a los cuidadores de sus otros hijos en el día de la consulta y 80 (39,6%) declaran pérdida de salario por la jornada laboral. De los casos de alta complejidad, el 9,33% de las madres entrevistadas habían abandonado su actividad remunerada habitual. Los gastos de transporte para una consulta de cirugía pediátrica en un hospital de referencia de Río de Janeiro cuestan de media el 4,42% del salario mínimo vigente, con una media de 217,32 minutos de tiempo de viaje/consulta. Los gastos de alimentación y la pérdida de salario por ausencia en el trabajo también implican una importante carga económica o pérdida de salario para el paciente en cada cita.

Nesta pesquisa, estudamos os custos de transporte por consulta ambulatorial em pacientes referenciados para avaliação cirúrgica especializada em um hospital terciário no Rio de Janeiro, Brasil. Trata-se de um estudo descritivo com responsáveis por pacientes pediátricos agendados para consulta de cirurgia pediátrica, questionando fatores ligados ao custo de transporte e de oportunidade (perda de remuneração, alimentação, pagamento de cuidadores para outros filhos, despesas para outros acompanhantes). Aproximadamente metade dos pacientes tinham até 5 anos de idade, cerca de 2/3 apresentavam doenças de resolutividade cirúrgica simples e definitiva e 181 famílias (89,17%) contavam com renda familiar mensal de até R$ 1.999,00. A proporção de famílias beneficiadas por transporte gratuito variou entre 4,26-15,56% para pacientes morando até 100km de distância do hospital (45,83% para pacientes com residência a mais de 100km da instituição). Dos responsáveis, 176 (87,13%) relataram despesas para alimentação, 12 (5,94%) pagavam cuidadores para os outros filhos no dia da consulta e 80 (39,6%) referiram perda do pagamento do dia de trabalho. Dos casos de alta complexidade, 9,33% das mães entrevistadas abriram mão de exercer atividade remunerada regular. As despesas com transporte para uma consulta em cirurgia pediátrica em um hospital de referência do Rio de Janeiro custam em média 4,42% do salário mínimo vigente, com uma média de 217,32 minutos de deslocamento por consulta. Despesas com alimentação e perda de remuneração pela ausência no trabalho também implicam encargos financeiros ou perda de remuneração significativos para o paciente em cada consulta.

ABSTRACT The analysis of transport demand is one of the main forms of public transport management and significantly impacts tax revenues and public management. This study evaluates the demand for intercity public transport in the Brazilian state of Rio Grande do Norte, from January 2019 to July 2022, in three categories of users: full tickets, half tickets, and gratuities. The hypothesis is that the integration of demand systems can identify underdeclaration bias by crossing declared demand data in the Transport Information Management System (GISIT) and the actual demand data from the Electronic Ticketing System obtained by the TDMax Reports software. Access to public transport databases was provided by the Departamento de Estradas de Rodagem do Rio Grande do Norte (DER/RN). The results reveal an underdeclaration of the demand for public transport in the state, observed in 41.74% of the fleet.

RESUMEN El análisis de la demanda de transporte es una de las principales formas de gestión del transporte público y provoca importantes impactos recaudatorios para la gestión pública. Así, este estudio tuvo como objetivo evaluar la demanda de transporte público interurbano en Rio Grande do Norte, de enero de 2019 a julio de 2022, en tres categorías de usuarios: boletos completos, medios boletos y gratuidades, a partir de la hipótesis de que la integración de los sistemas de demanda puede identificar el sesgo de declaración insuficiente al cruzar los datos de demanda declarados, presentes en el Sistema de gestión de información de transporte (GISIT), y los datos de demanda real del Sistema de emisión de boletos electrónicos, obtenidos por el software TDMax Reports. El acceso a las bases de datos de transporte público fue proporcionado por el Departamento de Carreteras de Rio Grande do Norte (DER/RN). Los resultados mostraron subdeclaración de la demanda de transporte público en RN, observada para el 41,74% de la flota.

RESUMO A análise da demanda de transportes é uma das principais formas de gerenciamento do transporte público e causa impactos arrecadatórios importantes para a gestão pública. Desse modo, este estudo objetivou avaliar a demanda do transporte público intermunicipal do Rio Grande do Norte, no período de janeiro de 2019 a julho de 2022, em três categorias de usuários: passagens inteiras, meias passagens e gratuidades, baseando-se na hipótese de que a integração dos sistemas de demanda pode identificar viés de subdeclaração ao cruzar dados declarados de demanda, presentes no Sistema de Gestão de Informações de Transportes (GISIT), e os dados reais de demanda do Sistema de Bilhetagem Eletrônica, obtidos pelo software TDMax Reports. O acesso aos bancos de dados do transporte público foi disponibilizado pelo Departamento de Estradas de Rodagem do Rio Grande do Norte (DER/RN). Os resultados demonstraram subdeclaração da demanda do transporte público do RN, observada para 41,74% da frota.

Resumo O artigo discute a escrita de biografias de mulheres cientistas a partir do relato das experiências de Evelyn Fox Keller, Georgina Ferry e Barbara Goldsmith na escrita de biografias das cientistas Barbara McClintock, Dorothy Hodgkin e Marie Curie, respectivamente. Discutimos como a escrita e a repercussão pública de tais biografias podem contribuir para uma reflexão teórica sobre as biografias de mulheres nas ciências. Consideramos ser necessário pensar a história das mulheres nas ciências como projetos marcadamente políticos, e, por isso, defendemos o conceito de biografias feministas.

Abstract This article discusses the writing of biographies of women scientists based on the experiences of Evelyn Fox Keller, Georgina Ferry and Barbara Goldsmith in writing the biographies of the scientists Barbara McClintock, Dorothy Hodgkin and Marie Curie, respectively. We discuss how the writing process and the public repercussions of these biographies contributes to a theoretical reflection on biographies of women in science. We consider it necessary to think of the history of women in science to be composed of clearly political projects, and for this reason defend the concept of feminist biographies.

RESUMO Avaliar a capacidade do obturador provisório (OP) e da gutapercha em bastão (GP) de prevenir a contaminação bacteriana dos condutos radiculares. Foram selecionados 80 incisivos centrais superiores decíduos artificiais que foram divididos aleatoriamente em 2 grupos: OP (n = 40) e GP (n = 40). Foi realizado acesso endodôntico, instrumentação rotatória, preenchimento do canal radicular e selamento coronário conforme os grupos. Foi feita a semeadura de S.mutans e E. faecalis na entrada do canal radicular. Ambos os grupos foram subdivididos em 5 tempos experimentais (24, 48, 72, 96 e 120 horas), com 8 espécimes por tempo experimental: 5 submetidos a preenchimento do canal radicular e selamento coronário (com OP ou GP) e 3 controles (apenas selamento coronário, sem preenchimento do canal). Todos os espécimes foram incubados em jarras de anaerobiose a 37°C e a contaminação bacteriana foi avaliada em espectrofotômetro. Utilizou-se ANOVA (teste t) para a comparação da contaminação e o teste de Kruskal-Wallis para a comparação dos escores da obturação entre os grupos experimentais. Foi observada diferença significativa no selamento nas primeiras 24 horas entre GP e controles (p = 0,046). Não houve diferença estatisticamente significativa no padrão de preenchimento entre os canais selados com OP versus GP. Os espécimes selados com GP apresentaram menor contaminação do que os controles nas primeiras 24 horas. Nos demais tempos experimentais, tanto GP quanto OP não foram eficientes no controle da contaminação bacteriana; ambos apresentaram falha no selamento coronário.

ABSTRACT To evaluate the ability of temporary cement (TC) and gutta-percha sticks (GP) to prevent bacterial contamination of the root canal through the coronal seal after pulpectomy. Eighty artificial primary maxillary central incisors were selected and randomly divided into 2 groups: TC (n = 40) and GP (n = 40). Endodontic access, rotary instrumentation, root canal filling, and coronal sealing were performed according to group allocation. The root canal opening was seeded with S. mutans and E. faecalis. Both groups were subdivided into 5 experimental time points (24, 48, 72, 96, and 120 hours), with 8 specimens per time-point group: 5 in which both root canal filling and coronal sealing were performed (with either TC or GP) and 3 controls (coronal sealing alone, without root canal filling). All specimens were incubated in an anaerobic jar at 37°C, and bacterial contamination was assessed in a spectrophotometer. ANOVA (t-test) was used to compare contamination and the Kruskal-Wallis test to compare filling scores between the experimental groups. A significant difference was observed in sealing in the first 24 hours between GP and controls (p = 0.046). There was no significant difference in the filling pattern between canals sealed with TC versus GP. Specimens sealed with GP showed less contamination than controls in the first 24 hours. At later time points, neither GP nor TC were effective at controlling bacterial contamination; both failed to provide adequate coronal sealing.

Abstract A Short-finned Pilot Whale, Globicephala macrorhynchus, was found dead and with shark bites along its body on a beach in northeast Brazil. The present study aimed to identify the shark species responsible for the bites as well as to estimate its/their total length. Species identification was performed using the bite marks, which were of the same diameter, suggesting they were infected by one or more Tiger sharks of similar size. The characteristics of the bites and the shape and distribution of the marks left by the teeth pointed to the Tiger Shark, Galeocerdo cuvier. The total length of the Tiger Shark or sharks was estimated at between 257 and 288 cm, based on the perimeter contour of each bite and the interdental distance inferred from the marks on the whale. This suggests that the bites were inflicted by one or more sub-adult specimens.

The Baltic German chemist Wilhelm Ostwald (1853-1932) had his scientific prestige eclipsed in the history of chemistry due to his rejection to atomic theory, which suggests the permanence of the whig interpretation on history of chemistry in the narratives about him. In this paper, we intend to present elements on Ostwald’s biography far from his role in the atomic controversy from the 19th century, as for example his efforts for the popularization of physical chemistry; his role as a teacher and a textbook writer, and his role as a chemical researcher, being responsible for relevant studies on chemical affinity and catalysis, for example. Finally, we conclude this paper defending that Ostwald’s trajectory cannot be neglected for those interested in history of science.

Resumo Objetivo relatar a experiência do uso da entrevista narrativa com pessoas em situação de rua com transtornos mentais. Método relato de experiência realizada em uma capital do Sul do Brasil. A coleta de dados ocorreu de julho a outubro de 2019. Resultados a aplicação da entrevista narrativa desenvolveu-se em duas etapas: aproximação com o campo e seleção dos participantes; e construção da narrativa. A primeira envolveu uma inserção em um Consultório na Rua e possibilitou a identificação e o desenvolvimento de vínculo com os usuários. A segunda ocorreu com o desenvolvimento da entrevista narrativa e envolveu os seguintes passos: contato e negociação, história principal e fase de questionamentos. Conclusão a entrevista narrativa se apresenta como ferramenta metodológica com potencial para a construção de itinerários terapêuticos de pessoas em situação de rua com transtornos mentais, contextualizando suas opções na busca pelo seu cuidado na perspectiva de suas histórias de vida. Implicação para a prática a entrevista narrativa possibilita fazer-se ouvir em histórias frequentemente negligenciadas. Por ter como foco central a construção de sentidos por parte de quem vivencia determinada realidade, permitiu a reconstrução desses, possibilitando novos olhares e insights em relação à própria experiência de quem relatava.

Abstract Objective report the experience of using the narrative interview with homeless persons who suffer from mental disorders. Method experience report held in a capital city in the South of Brazil. The data collection took place from July to October 2019. Results the application of the narrative interview was developed in two stages: approach to the field and selection of participants; and construction of the narrative. The first involved an insertion in a Street Office and made it possible to identify and develop a link with the users. The second occurred with the development of the narrative interview and involved the following steps: contact and negotiation, main story and questioning phase. Conclusion the narrative interview presents itself as a methodological tool with potential for the construction of therapeutic itineraries for homeless persons with mental disorders, contextualizing their options in the search for their care from the perspective of their life stories. Implication for practice narrative interviewing makes it possible to make oneself heard in often neglected stories. By having as a central focus the construction of senses by those who experience a certain reality, it allowed the reconstruction of these, allowing new looks and insights in relation to the very experience of those who reported.

Resumen Objetivo relatar la experiencia de utilizar entrevistas narrativas con personas en situación callejera con trastornos mentales. Método informe de experiencia realizado en una capital del Sur de Brasil. La recolección de datos tuvo lugar de julio a octubre de 2019. Resultados La aplicación de la entrevista narrativa se desarrolló en dos etapas: aproximación al campo y selección de participantes; y construcción de la narrativa. La primera implicó una inserción en un Consultório na Rua y permitió identificar y desarrollar vínculos con los usuarios. La segunda ocurrió con el desarrollo de la entrevista narrativa y contó con los siguientes pasos: contacto y negociación, historia principal y fase de cuestionamientos. Conclusión la entrevista narrativa se presenta como una poderosa herramienta metodológica para la construcción de itinerarios terapéuticos para personas en situación callejera con trastornos mentales, contextualizando sus opciones en la búsqueda de su cuidado desde la perspectiva de sus historias de vida. Implicación para la práctica la entrevista narrativa permite hacerse oír en historias que a menudo se pasan por alto. Debido a que tiene como foco central la construcción de significados por parte de quienes experimentan una determinada realidad, permitió la reconstrucción de estos, posibilitando nuevas miradas y percepciones en relación a la propia experiencia de quien informa.

Abstract Purpose: To evaluate clinically dogs that underwent tibial tuberosity advancement (TTA) six months previously. Methods: Dogs of various breeds, gender, weight, and age that had CCL rupture and underwent TTA for treatment were included in this study. Parapatellar arthrotomy was performed in all patients to assess the joint for a ruptured ligament and meniscal injury before the TTA. The appropriate cage for the TTA was chosen with planning surgery. The surgical procedure was performed according to the literature, using a modified Maquet technique. Six months after surgery, lameness during walking; muscular atrophy; crepitation, cranial drawer and tibial compression tests and quality of life based on owner’s evaluation were assessed. Results: Postoperative complications were observed in only one knee (4.76%), with a surgical site seroma. The mean lameness score at walking was 0.29 (± 0.64). The mean score regarding muscular atrophy was 0.95 (± 1.56). The mean score of the cranial drawer test, in a range from 0 to 5, was 1.52 (± 1.54). The owners rated the dog’s quality of life as excellent in 44%, good in 30%, and moderate in 17%. Conclusion: This clinical study supports the affirmation that patients who undergo TTA for treatment of CCL rupture have an acceptable response.

Resumen 27. El objetivo de este estudio fue describir la prevalencia del Síndrome de Burnout entre los profesores de la Escuela Estatal en Niterói, Brasil. Se trata de un estudio cuantitativo, descriptivo, con información de una encuesta realizada con maestros. El cuestionario utilizado constó de preguntas cerradas que abordaron las características sociodemográficas y las características del trabajo de los sujetos de investigación y el Maslach Burnout Inventory. La sospecha de prevalencia de síndrome de burnout según criterio de Grunfeld fue de 33 casos (63.5%). La prevalencia significativa de este síndrome entre los maestros genera alerta sobre las condiciones de trabajo y de la salud mental de estos profesionales. Se concluye que, a partir de los datos analizados, se observó que la profesión carece de intervenciones para mejorar la calidad de vida de los docentes en su trabajo.

Resumo 23. O objetivo deste estudo foi descrever a prevalência da Síndrome de Burnout entre os professores da Escola Estadual em Niterói, Brasil. É um estudo quantitativo e descritivo, com informações de uma pesquisa realizada com professores. O questionário utilizado consistiu em questões fechadas que abordavam as características sociodemográficas e as características de trabalho dos sujeitos da pesquisa e o Inventário Maslach Burnout. A suspeita de prevalência de síndrome de burnout de acordo com os critérios de Grunfeld foi de 33 casos (63,5%). A prevalência significativa desta síndrome entre os professores gera alerta sobre as condições de trabalho e a saúde mental desses profissionais. Conclui-se que, a partir dos dados analisados, observou-se que a profissão não possui intervenções para melhorar a qualidade de vida dos professores em seus trabalhos.

Abstract 31. The objective of this study was to describe the prevalence of Burnout Syndrome among the professors of the State School in Niterói, Brazil. It is a quantitative, descriptive study, with information from a survey conducted with teachers. The questionnaire used consisted of closed questions that addressed the sociodemographic characteristics and work characteristics of the research subjects and the Maslach Burnout Inventory. Suspected prevalence of burnout syndrome according to Grunfeld's criteria was 33 cases (63.5%). The significant prevalence of this syndrome among teachers generates alert about working conditions and the mental health of these professionals. It is concluded that, from the data analyzed, it was observed that the profession lacks interventions to improve the quality of life of teachers in their work.

ABSTRACT BACKGROUND: Chronic lung infections, inflammation and depletion of nutritional status are considered to be prognostic indicators of morbidity in patients with cystic fibrosis. The aim of this study was to investigate the association between inflammatory markers and lung function, nutritional status and morbidity among children/adolescents with cystic fibrosis. DESIGN AND SETTINGS: Prospective three-year longitudinal study conducted in an outpatient clinic in southern Brazil. METHODS: Children/adolescents aged 1-15 years with cystic fibrosis were enrolled. Nutritional status was determined from weight-to-length and body mass index-to-age z-scores and was classified as acceptable, at risk or nutritional failure. Tumor necrosis factor-α, interleukin-1β, myeloperoxidase, C-reactive protein and C-reactive protein/albumin ratio were analyzed. Lung function was evaluated based on the forced expiratory volume in the first second and morbidity according to the number of hospitalizations for pulmonary exacerbation and infections by Pseudomonas aeruginosa. Lung function, nutritional status and morbidity were the outcomes. Odds ratios and 95% confidence intervals were to evaluate the effect of baseline inflammatory markers on the clinical outcomes after three years of follow-up and p-values < 0.05 were considered significant. RESULTS: We evaluated 38 children/adolescents with cystic fibrosis: 55% female; median age (with interquartile range), 3.75 years (2.71-7.00). Children/adolescents with high C-reactive protein/albumin ratio at baseline had odds of 18 (P = 0.018) of presenting forced expiratory volume in the first second ≤ 70% after three years. The other inflammatory markers were not associated with the outcomes. CONCLUSION: C-reactive protein/albumin ratio was associated with forced expiratory volume in the first second ≤ 70% after three years.

RESUMO: O objetivo deste trabalho foi avaliar o efeito da utilização do óleo essencial de alho (Allium sativum) sobre as taxas de fertilização dos ovos e eclosão e normalidade de larvas de jundiá cinza, Rhamdia quelen, incubados artificialmente. O experimento foi realizado utilizando delineamento experimental inteiramente casualizado com quatro tratamentos e cinco repetições. Os tratamentos consistiram na introdução de óleo essencial de alho nas incubadoras, utilizando um sistema fechado de recirculação de água, com concentrações de 0; 1; 3 e 5mg L-1 de óleo essencial de alho. As taxas de fertilização dos ovos e eclosão das larvas foram determinadas, respectivamente as 10 e 24h após o início da hidratação dos ovos. Utilizou-se do padrão morfológico de larvas para determinar a taxa de normalidade, com o auxílio de um microscópio estereoscópio. A concentração de 5mg L-1 promoveu melhores taxas de fertilização de ovos e eclosão de larvas. As concentrações de óleo essencial de alho apresentaram efeito linear sobre as taxas de fertilização dos ovos e eclosão das larvas. O óleo de alho não influenciou na normalidade das larvas (P>0,05). Recomenda-se utilizar 5mg L-1 de óleo essencial de alho para promover melhores taxas de fertilização de ovos e eclosão de larvas de jundiá cinza incubados artificialmente.

ABSTRACT: The objective of this study was to evaluate the effects of garlic (Allium sativum) essential oil on the rates of eggs fertilization and hatching and on the normal development of larvae of artificially incubated silver catfish (Rhamdia quelen). The experiment was carried out using a completely randomized experimental design with four treatments and five replicates. The treatments consisted of introducing garlic essential oil into the incubators, which used a closed system of water recirculation, at concentrations of 0, 1, 3, and 5mg L-1. Rates of eggs fertilization and larval hatching were determined at 10 and 24h after the beginning of eggs hydration. Morphology of larvae was assessed with the aid of a stereo microscope to determine the rate of normal development. The concentration of 5mg L-1 promoted better rates of eggs fertilization and larval hatching. Concentrations of garlic essential oil had a linear effect on eggs fertilization rates and larval hatching. Garlic oil did not influence the morphology of the larvae (P>0.05). It is recommended to use 5mg L-1 of garlic essential oil to promote better rates of eggs fertilization and larval hatching of artificially incubated silver catfish.

Abstract The core microbiota of a neutral mine drainage and the surrounding high heavy metal content soil at a Brazilian copper mine were characterized by 16S rDNA pyrosequencing. The core microbiota of the drainage was dominated by the generalist genus Meiothermus. The soil samples contained a more heterogeneous bacterial community, with the presence of both generalist and specialist bacteria. Both environments supported mainly heterotrophic bacteria, including organisms resistant to heavy metals, although many of the bacterial groups identified remain poorly characterized. The results contribute to the understanding of bacterial communities in soils impacted by neutral mine drainage, for which information is scarce, and demonstrate that heavy metals can play an important role in shaping the microbial communities in mine environments.

OBJETIVO: investigar os aspectos da vida familiar que influenciam o desempenho linguístico de crianças com síndrome de Down. MÉTODOS: aplicação do questionário Recursos do Ambiente Familiar e análise pragmática da comunicação de crianças com síndrome de Down. Participaram do estudo 30 crianças com SD, de cinco a 10 anos de idade e de ambos os sexos. Aplicou-se o teste de correlação de postos de Spearman. As análises foram feitas pelo programa SPSS versão 10.0 e, em todas elas, adotou-se um nível de significância de 5%. RESULTADOS: verificou-se correlação entre itens do questionário e os resultados referentes ao meio comunicativo e função comunicativa da análise pragmática utilizadas pelas crianças. CONCLUSÃO: é preciso considerar os aspectos do meio familiar que apresentam influência com o desempenho comunicativo de crianças com Sindrome de Down.

PURPOSE: to investigate the aspects of family life that influence communicative performance of children with Down syndrome. METHODS: application of the "Features of the Family Environment" questionnaire and pragmatic analysis of children with DS communication. 30 children of both genders, ranging from five to 10 years old, have partaken this research. Spearman's rank order correlation was applied. The analyses were performed using the SPSS' 10.0 version, and in all of them, a significance level of 5% was adopted. RESULTS: correlation between the items of the questionnaire and pragmatic analysis results regarding communicative means and communicative function was found to exist. CONCLUSION: it is necessary to take family life aspects that influence the communicative performance of children with DS into consideration.